Norovirus RT-PCR

Norovirus PCR

BioFire Gastrointestinal Panel

Molecular

Virology

Reverse Transcriptase - Polymerase Chain Reaction (RT-PCR)

BioFire FilmArray Multiplex PCR

Raw stool/vomitus specimen in sterile cup.  No additive or medium

Stool in Cary-Blair media

Norovirus Specimen Collection

Store/transport refrigerated (2-8 °C).

For BioFire GI Panel specimens need to be received by the laboratory within 4 days of collection.

Stool (with and without Cary-Blair)
Vomitus

Virology Test Request Form

• RT-PCR Results
  • Norovirus GI Positive
  • Norovirus GII Positive
  • Norovirus Negative
• BioFire Results
• Not Detected
• Test Cancelled
• Detected
  • Bacteria
    • Campylobacter (C. jejuni / C. coli / C. upsaliensis)
    • Clostridioides (Clostridium) difficile (toxin A/B)
    • Plesiomonas shigelloides
    • Salmonella
    • Yersinia enterocolitica
    • Vibrio (V. parahaemolyticus / V. vulnificus / V. cholerae)
      • Vibrio cholerae
  • Diarrheagenic Eschericia Coli/Shigella
    • Enteroaggregative E. coli (EAEC)
    • Enteropathogenic E. coli (EPEC)
    • Enterotoxigenic E. coli (ETEC) lt/st
    • Shiga-like toxin-producing E. coli (STEC) stx1/stx2
    • E. coli O157
    • Shigella/Enteroinvasive E. coli (EIEC)
  • Parasites
    • Cryptosporidium
    • Cyclospora cayetanensis
    • Entamoeba histolytica
    • Giardia lamblia
  • Viruses
    • Adenovirus F40/41
    • Astrovirus
    • Norovirus GI/GII
    • Rotavirus A
    • Sapovirus (I, II, IV, and V)

Specimen not identified

Not meeting laboratory testing criteria
Incorrect specimen type

Stool/vomitus specimens are likely to contain substances that will interfere with RT-PCR assays.

Multiple specimens (3-10 individuals) are requested to maximize the likelihood of positive test results.

One working day

N/A

N/A

N/A

Stool specimens in Cary-Blair will also be tested using the BioFire GI panel.