Measles

Brief Description

Molecular testing for the detection of Measles Virus in clinical samples. This testing has the ability to differentiate between measles wild-type viruses and the vaccine strain found in the Measles, Mumps, and Rubella (MMR) vaccine.

Laboratory Unit

Molecular

Methodology

Real-Time Reverse Transcription Polymerase Chain Reaction (rRT-PCR)

Specimen Collection

Collection and Submission of Measles Specimens for Real-Time PCR Testing

Storage/Transport

Collection and Submission of Measles Specimens for Real-Time PCR Testing

Specimens should be stored refrigerated (2-8°C). Send immediately to MSPHL on freezer packs. If specimens will not arrive at the MSPHL within 10 days of collection, specimens should be stored frozen (≤-20°C) and shipped on dry ice.

Acceptable Specimen Type(s)

Collection and Submission of Measles Specimens for Real-Time PCR Testing

Detection of measles virus is most sensitive when respiratory specimens are collected within the first 3 days of rash onset. If unable to collect a respiratory specimen within 72 hours of onset, urine specimens may be collected in addition to respiratory specimens. Collection of a respiratory specimen along with a urine sample may improve test sensitivity, especially when collecting toward the end of the RT-PCR collection window.

If collected within 72 hours of symptom onset collect one of the following:

  • Throat Swab
  • Nasopharyngeal Swab

If collected between 4-10 days of symptom onset, collect both of the following:

  • A respiratory sample (either throat or nasopharyngeal swab)
  • Urine Sample

Test Request Form(s)

Virology Test Request Form

Possible Results

  • Measles Virus RNA Not Detected
  • Measles Wild-Type Infection Indicated
  • Measles Vaccine Infection Indicated
  • Measles Virus RNA Detected, Genotype Undetermined
  • Inconclusive
  • Invalid

Unacceptable Conditions

Improperly identified (e.g. missing patient identifiers)

Not meeting laboratory testing criteria

Interfering Substances

Use only viral transport medium and synthetic Dacron/flocked swab or equivalent.  Do not use wooden shaft or cotton swab.

Result Reported

Measles Virus PCR results are typically reported within one business day.

Fees

N/A

CPT Codes

87798

LOINC/SNOMED Codes

N/A

Additional Information

Collection and Submission of Measles Specimens for Real-Time PCR Testing

CDC Measles Information