E. coli Bacteria Enumeration in Rec & Env Waters
Recreational/Environmental water test that enumerates E. coli bacteria using defined enzyme substrate technology.
Standard Method 9223B Enzyme Substrate Most Probable Number (MPN), using Idexx Colilert 24-hour QuantiTray format.
Sterile, disposable 120 ml plastic bottle containing sodium thiosulfate. Must use sample kit provided by MSPHL.
Ship to MSPHL immediately. Must be received in lab within 48 hours of sampling. May be stored in refrigerator conditions temporarily prior to shipping. Use of a cooler and ice packs in the field is recommended.
Acceptable Specimen Type(s)
Recreational waters from public pools, hotel pools and spas, and swim beaches. Environmental samples include non-recreational surface waters submitted by a state or local health agency for investigation.
St. Louis Zoo animal tanks and pools are also tested.
Test Request Form(s)
Use the Recreational/Environmental Water Sample Form.
E. coli MPN/100 ml. (Method can also give a Total Coliform MPN/100 ml if appropriate).
E. coli <235 per 100 ml.
Transit time exceeded
No date/time collected
No sample identification
Sample form incomplete
Quantity not sufficient (100 ml)
Sample leaked in transit
P resence of disinfectant
Humic material with innate yellow color
Results are reported to the submitter within 24-30 hours of sample receipt in lab (next day).
Samples are charged a $20 fee. Invoice will be sent with sample results. Testing is performed on a contractual basis with a federal or state agency, or with privately owned entities (homeowners' associations). Contracts cannot be formed with individuals to test privately owned pools or beaches.
Recreational/environmental water samples must be submitted by an official of the state or local health agency, or a representative of the contractee. Use the Recreational/Environmental Water