Norovirus RT-PCR

Brief Description

Norovirus PCR

BioFire Gastrointestinal Panel

Laboratory Unit

Molecular

Virology

Methodology

Reverse Transcriptase - Polymerase Chain Reaction (RT-PCR)

BioFire FilmArray Multiplex PCR

Specimen Collection

Raw stool/vomitus specimen in sterile cup.  No additive or medium

Stool in Cary-Blair media

Storage/Transport

Store/transport refrigerated (2-8 °C).

For BioFire GI Panel specimens need to be received by the laboratory within 4 days of collection.

Acceptable Specimen Type(s)

Stool (with and without Cary-Blair)
Vomitus

Test Request Form(s)

Virology Test Request Form

Possible Results
  • RT-PCR Results
    • Norovirus GI Positive
    • Norovirus GII Positive
    • Norovirus Negative
  • BioFire Results
    • Not Detected
    • Test Cancelled
    • Detected
      • Bacteria
        • Campylobacter (C. jejuni / C. coli / C. upsaliensis)
        • Clostridioides (Clostridium) difficile (toxin A/B)
        • Plesiomonas shigelloides
        • Salmonella
        • Yersinia enterocolitica
        • Vibrio (V. parahaemolyticus / V. vulnificus / V. cholerae)
          • Vibrio cholerae
      • Diarrheagenic Eschericia Coli/Shigella
        • Enteroaggregative E. coli (EAEC)
        • Enteropathogenic E. coli (EPEC)
        • Enterotoxigenic E. coli (ETEC) lt/st
        • Shiga-like toxin-producing E. coli (STEC) stx1/stx2
          • E. coli O157
        • Shigella/Enteroinvasive E. coli (EIEC)
      • Parasites
        • Cryptosporidium
        • Cyclospora cayetanensis
        • Entamoeba histolytica
        • Giardia lamblia
      • Viruses
        • Adenovirus F40/41
        • Astrovirus
        • Norovirus GI/GII
        • Rotavirus A
        • Sapovirus (I, II, IV, and V)
Unacceptable Conditions

Specimen not identified

Not meeting laboratory testing criteria
Incorrect specimen type

Interfering Substances

Stool/vomitus specimens are likely to contain substances that will interfere with RT-PCR assays.

Multiple specimens (3-10 individuals) are requested to maximize the likelihood of positive test results.

Result Reported

One working day

Fees

N/A

CPT Codes

N/A

LOINC/SNOMED Codes

N/A

Additional Information

Stool specimens in Cary-Blair will also be tested using the BioFire GI panel.