Zika Virus

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Brief Description

Real-Time PCR testing for Zika Virus, Chikungunya Virus, and Dengue Virus by the CDC Trioplex rRT-PCR Assay, verified for use under Emergency Use Authorization by the FDA.

ELISA (Enzyme-Linked ImmunoSorbent Assay) IgM serology testing for Zika (verified for use under Emergency Use Authorization by the FDA), Chikungunya, and Dengue

Laboratory Unit

Molecular and Virology

Methodology

PCR and IgM Serology testing is done in accordance with CDC guidelines for laboratories testing for Zika Virus.

CDC lab guidance: http://www.cdc.gov/zika/laboratories/lab-guidance.html

Note: Testing for Zika, Chikungunya, and Dengue is available only after consultation with the Bureau of Communicable Disease Control and Prevention at (573) 751-6113 or after hours 800-392-0272.

Specimen Collection

Zika collection kit contains one (1) red-top blood collection tube, one (1) urine collection cup, parafilm for sealing urine cup, and two (2) freeze pillows.

PCR and/or IgM Serology:

  • Collect a serum and urine specimen from all patients. NOTE: URINE MUST ACCOMPANY A PATIENT-MATCHED SERUM. Testing cannot be performed if only a urine specimen is submitted.
  • Collect blood in a red top or marble-top (serum separator) Vacutainer tube. Centrifuge the blood and transfer the serum to a new red top or other sterile tube. At least 1 ml of serum is required for PCR and serological testing. At least 1 mL of urine is required for PCR testing.

Storage/Transport

Store refrigerated until transport, pack specimen kit with frozen cold pack for transport. For fastest delivery, ship specimens by MSPHL Courier. For listing of courier stops, click here.

Acceptable Specimen
Type(s)

Serum and patient-matched urine




Test Request Form(s)

Virology Test Request Form

Possible Results

RT-PCR PCR
No Zika, Dengue, or Chikungunya RNA detected by rRT-PCR

Dengue RNA Detected by rRT-PCR
Chikungunya RNA Detected by rRT-PCR
Zika RNA Detected by rRT-PCR
Specimen Inconclusive for the presence of Zika, Dengue, and Chikungunya RNA by rRT-PCR. An inconclusive result may occur in the case of an inadequate specimen.

IgM Serology
Zika Virus ELISA IGM: Positive: Presumptive Zika Virus. Specimen forwarded to the CDC for further testing.
Chikungunya Virus ELISA IGM: Positive: Presumptive Chikungunya Virus. Specimen forwarded to the CDC for further testing.
Dengue Virus ELISA IGM: Positive: Presumptive Dengue Virus. Specimen forwarded to the CDC for further testing.
Negative
Equivocal, Specimen forwarded to the CDC for further testing.
Indeterminate
Unsatisfactory
Uninterpretable
Unacceptable Specimen

Reference Range

PCR: Detected, Not Detected, or Inconclusive
IgM serology: Positive presumptive, Negative, or Equivocal.

Unacceptable Conditions

Specimen not properly labeled
Quantity Not Sufficient for testing
Hemolyzed specimen

Urine specimen received without patient-matched serum

Interfering Substances

N/A


Result Reported

PCR: 1-4 days
IgM Serology: 1-9 days

Fees

N/A

CPT Codes

N/A

LOINC/SNOMED Codes

N/A