HIV-1 with Reflex to HIV-1 Confirmation by Western Blot

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Brief Description

Specimens will be screened by an Enzyme Immunoassay (EIA) test that uses HIV-1 antigens, including inactivated, purified HIV-1 viral lysate proteins, for the detection of antibodies to HIV-1.  Any repeatedly reactive EIA specimen will be confirmed with a Western Blot test that detects and identifies individual proteins to Human Immunodeficiency Virus Type 1 (HIV-1).  The Western Blot is considered a more specific test than the screening test.  This test is only available to program approved sites.

Laboratory Unit

Immunology

Methodology

Enzyme Immunoassay/Western Blot

Specimen Collection

OraSure Collection Device:  Place the pad in the mouth (pad oriented down) between the lower cheek and gum, and gently rub the pad back and forth along the gum line until the pad is moist.  Leave the pad in the mouth for a minimum of two minutes and a maximum of five minutes.  At the end of two minutes, remove the pad from mouth, insert the pad into the vial, and push it all the way to the bottom of the vial into the blue liquid.  Break the plastic shaft by bending it against the side of the vial until the upper half snaps off.  Replace the cap tightly.  Specimen must be received within 21 days of collection.

Storage/Transport

Store specimens at refrigerator temperature (2-8° C) prior to shipment.
Specimens should be sent daily or next working day.
Transport in MSPHL shipping containers at room temperature.

Acceptable Specimen
Type(s)

Oral mucosal transudate



Test Request Form(s)

Immunology Test Request Form

Possible Results

EIA:  Reactive, Non-Reactive, Unsatisfactory
Western Blot:  Positive, Negative, Indeterminate

Reference Range

HIV 1 EIA:  Non-Reactive
OraSure HIV-1 Western Blot:  Negative

Unacceptable Conditions

No Name on Specimen
Name on Specimen Does Not Match Name on Request Form
Quantity Not Sufficient
Collection Device Expired
Receipt of Specimen > 21 days After Collection Date

Interfering Substances

N/A

Result Reported

7-8 days after receipt of specimen, specimens are batched and ran once weekly

Fees

N/A

CPT Codes

86701 EIA; if reflexed, add 86689 Western Blot

LOINC/SNOMED Codes

EIA:  35437-3
Western Blot:  35439-9

Additional Information

Testing alone cannot be used to diagnose AIDS, which is a clinical syndrome.  The diagnosis of AIDS must be established clinically.  A negative test result at any point does not preclude the possibility of exposure to or infection with HIV-1 and/or HIV-2.

Add the sentences (Effective April 1, 2012, the HIV Blue Card, officially known as the Provider's Confidential Report of HIV/AIDs Infection report form (MO 580-1641), will no longer be provided with the positive HIV Western Blot laboratory results. Click here to view letter.)